Data and Finished Chromosomes

Learn more about the design and experimental characterization of each finished chromosomes. The GFF3 version includes the annotation and the FASTA sequence record. The WT version is for the S288c reference strain and the synthetic version is the final design target.

Overview

status

Reference sequence (17 MB sequence plus annotation): Saccharomyces cerevisiae S288C genome (version=R64-2-1) created by the Saccharomyces Genome Database

Detailed Status (Nov 2023)

Chromosome Teams   WT GFF3   Synthetic GFF3 Publication
I NYU 230 MB chr01 181 MB syn01 Synthetic chromosome fusion: effects on mitotic and meiotic genome structure and function (synI)
II Edinburgh + BGI 813 MB chr02 770 MB syn02 Deep functional analysis of synII, Shen et al 2017 Science
III JHU 317 MB chr03 272 MB syn03 Total synthesis of a functional designer eukaryotic chromosome, Annaluru et al 2014 Science (synIII)
IV NYU + JGI + TITech + SIAT 1532 MB chr04 1455 MB syn04 Manipulating the 3D organization of the largest synthetic yeast chromosome (synIV)
V Tianjin 577 MB chr05 536 MB syn05 “Perfect” designer chromosome V, Xie et al 2017 Science
VI JHU + NYU + GenScript 270 MB chr06 243 MB syn06 synVI and beyond, Mitchell et al Science 2017
VII Manchester + BGI 1091 MB chr07 1029 MB syn07 Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast
VIII NYU 563 MB chr08 507 MB syn08 Context-dependent neocentromere activity in synthetic yeast chromosome VIII
IX JHU 440 MB chr09 406 MB syn09 IXR: Synthetic chromosome arms function in yeast, Dymond et al 2011 Nature IX: Consequences of a telomerase-related fitness defect and chromosome substitution technology in yeast synIX strains
X Tianjin 746 MB chr10 707 MB syn10 Chemically synthetized chromosome X, Wu et al 2017 Science
XI Imperial College 667 MB chr11 660 MB syn11 Synthetic yeast chromosome XI design provides a testbed for studying extrachromosomal circular DNA
XII Tsinghua 1078 MB chr12 999 MB syn12 Engineering the ribosomal DNA in a megabase synthetic chromosome (synXII), Zhang et al 2017 Science
XIII BGI + SIAT 924 MB chr13 884 MB syn13 synXIII to come
XIV Macquarie + Australian Wine Research Inst 784 MB chr14 753 MB syn14 Parallel laboratory evolution and rational debugging reveal genomic plasticity to Saccharomyces cerevisiae synthetic chromosome XIV defects and rearrangements
XV National U Singapore 1091 MB chr15 1048 MB syn15 Establishing Chromosomal Design-Build-Test-Learn through Combinatorial Reconfiguration of a Synthetic Chromosome (synXV)
XVI Macquarie 948 MB chr16 903 MB syn16 synXVI to come
neo-tRNA Manchester Does not exist! 190 KB Design, construction, and functional characterization of a tRNA neochromosome in yeast
Multi Combined Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions